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In vitro methylation protection. Squares represent the percentage of DNA in a complex with protein, as determined by EMSA, and circles represent the percentage of methylated DNA, as determined by an in vitro methylation protection assay (see Materials and Methods). (A) Data obtained with pap regulatory region DNA and purified Lrp. (B) Data obtained with gutABD regulatory region DNA and CAP. (C) Data obtained with gutABD regulatory region DNA and GutR.
