FIG. 2.

DNA methylation pattern analyses. The DNA methylation states of GATC-44.5 of the gutABD operon in isolate DL1882 (lanes 1 to 6) and isogenic gutR isolate DL2803 (lanes 7 to 12) were examined as described in Materials and Methods. Odd-numbered lanes show DNAs digested with BamHI and Acc65I, and even-numbered lanes show DNAs digested with BamHI, Acc65I, and MboI. DNA was isolated from cultures grown with glucose (lanes 1, 2, 7, and 8), glycerol (lanes 3, 4, 9, and 10), or glucitol (lanes 5, 6, 11, and 12) in the logarithmic phase of growth. DNA fragments A and E were from the gutA′-lacZ fusion, and DNA fragments B, C, and D were from the wild-type gutABD operon.