TABLE 2.
Transcription of chromosomal gutA′-lacZ fusions during growth with various carbon sourcesa
| Strain | Relevant genotype | Carbon source | β-Galactosidase activity (Miller units)b |
|---|---|---|---|
| DL1882 | gutA′ (GATC)-lacZ | Glucose | 89 ± 4.3 |
| Glycerol | 435 ± 27 | ||
| Glucitol | 4,159 ± 108 | ||
| DL2803 | gutA′ (GATC)-lacZ gutR | Glucose | 953 ± 127 |
| Glycerol | 4,289 ± 145 | ||
| Glucitol | 3,006 ± 197 | ||
| DL2453 | gutA′ (GATT)-lacZ | Glucose | 47 ± 3 |
| Glycerol | 202 ± 8.1 | ||
| Glucitol | 2,300 ± 80 |
a
The β-galactosidase level was measured in gutR+ isolate DL1882, gutR isolate DL2803, and DL2453, containing a GATT substitution at GATC-44.5. Cultures were grown with glucose, glycerol, or glucitol as the sole carbon source.
b
Error is expressed as the standard deviation of the mean for at least two independent cultures with three replicates each.