Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 30;3(2):164–173. doi: 10.1038/s43588-022-00375-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a, Biophysical model for mixtures of stem antibodies binding competitively to an HA monomer. b, Stem + stem mixtures were measured against the virus panel. Mixture IC₅₀ values are predicted using the neutralization of each individual antibody. The gray shading represents ≤4-fold error, where 1-fold error represents an exact prediction. c, Regions of ≥50% neutralization for a two-antibody mixture (outlined in solid lines) versus the neutralization of each individual antibody (dashed lines). The legend shows the concentration of each antibody in the mixture. d, Using a mixture’s neutralization titers, we predict the number, stoichiometry and neutralization profiles of the stem antibodies within. One such stem + stem mixture is shown (gray antibodies, mixture 8 in Supplementary Fig. 10), together with the predicted decomposition (red). Circles around each antibody represent ≥50% virus neutralization when the total antibody concentration is 10^−8.5 M, while factoring in antibody stoichiometry within the mixture (Methods). Average error represents the fold-difference between the collective neutralization predicted by the inferred stem antibodies and the measured neutralization of only the stem antibodies in the mixture, with 〈error〉 = 1 representing exact predictions. e, Mean + s.e.m. for the decomposition of 27 monoclonal antibodies, 11 mixtures containing two antibodies (stem + stem or head + stem) and three mixtures containing three antibodies (head + head + stem or head + stem + stem). The fractions of decompositions that predicted the correct number of stem antibodies are shown above each bar. Experimental noise is ~2-fold (dashed line). f, Examples showing additional mixtures combining two stem antibodies with and without a head antibody (additional decompositions are shown in Supplementary Fig. 10). Neutralization of two nearby antibodies is expanded because either can neutralize a virus, analogous to the solid lines in c. The insets at the top left show the number of head (brown) and stem (gray) antibodies in each mixture.

Source data