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. 2022 Dec 29;3(1):59–70. doi: 10.1038/s43588-022-00389-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Isothermogram representing the titration of hFGF1 with heparin hexasaccharide. a, The raw data. The horizontal red line represents the zero axis in the plot. b, The best fit of the raw data (one set of sites binding model). In the specific experiment shown, K_d is 1.68 ± 0.03 μM and the free energy from experimental K_d is −7.88 ± 0.01 kcal mol⁻¹. ΔH, heat change.

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