Fig. 1.
p-tau Ser356 expression increases in a Braak stage-dependent manner in postmortem AD temporal cortex. a Representative Western blot image of total homogenates (TH) and synaptoneurosomes (SN) from Braak 0–I, III–IV or VI (diagnosed AD) stage postmortem brain. Blots were probed for total protein (REVERT stain), total tau (Tau-5) and p-tau Ser356. b There is no significant impact of Braak stage (F(2,46.01) = 1.32, p = 0.28) or preparation (TH v SN) (F(1,27.00) = 0.15, p = 0.71) on the levels of total tau (Tau-5) in human postmortem brain. c There is a significant increase in p-tau Ser356 (normalised to total tau) with increasing Braak stage (**F(2,42.28) = 5.72, p = 0.006), with a significant difference between Braak 0–I and Braak VI AD (***t(27) = 4.13, p = 0.0009) and a strong trend increase between Braak III–IV and Braak VI (t(27) = 2.45, p = 0.053). There is an effect of preparation (*F(1,27.00) = 4.94, p = 0.035), with increased p-tau Ser356 in the total homogenate relative to synaptoneurosomes in Braak VI AD postmortem brains (*t(27) = 2.23, p = 0.034). Each point on the graphs represents a single case, triangles = males, circles = females. N = 10 cases per Braak stage