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. 2024 Jan 4;15:262. doi: 10.1038/s41467-023-44414-5

Fig. 6. Syt1 forms droplets in HEK293T cells and cultured rat hippocampal neurons.

Fig. 6

a Three representative super-resolution fluorescence images of HEK293T cells overexpressing syt1 C2AB (80–421)-GFP showing protein droplet formation 24-48 h post-transfection (Scale bars, 10 µm). b Same as a but with overexpressed syt1 C2AB (143–421)-GFP, syt1 C2AB (80 JuxtaK-421)-GFP, and cytoGFP. These constructs fail to form protein droplets (Scale bars, 10 µm). c Representative super-resolution fluorescence images of rat hippocampal neurons overexpressing transfected syt1 C2AB (80–421)-GFP, syt1 C2AB (143–421)-GFP, and syt1 C2AB (80 JuxtaK-421)-GFP. The first construct forms protein droplets, whereas the other two fail to do so; n = 3 (Scale bars, 10 µm). d Immunoblot of HEK293T cell lysates with WT and the overexpressed syt1 C2AB constructs described in a, stained with an anti-GFP antibody. β-actin served as a loading control. e Immunoblot to estimate syt1 C2AB (80–421)-GFP expression levels in HEK293T cells compared to endogenous syt1 in cultured rat hippocampal neuronal lysates, probed using an anti-syt1 antibody. β-actin again served as a loading control; n = 2. Uncropped blots and source data are provided as a Source Data file.