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Dissociation constants (KD values) for Ca2+ binding to the indicated syt1 cytoplasmic domain constructs, measured using ITC
| Dissociation constant | syt1 C2AB (80–421) | syt1 C2AB (96–421) | syt1 C2AB (80 JuxtaK-421) |
|---|---|---|---|
| # binding sites | 5 | 5 | 4 |
| KD1 (µM) | 80.0 ± 3.9 | 23.5 ± 2.1 | 14.1 ± 2.5 |
| KD2 (µM) | 190 ± 25 | 210 ± 89 | 24.2 ± 4.4 |
| KD3 (µM) | 309 ± 53 | 250 ± 110 | 156 ± 19 |
| KD4 (µM) | 1310 ± 125 | 451 ± 100 | 925 ± 150 |
| KD5 (µM) | 2900 ± 321 | 2510 ± 430 |
Data are mean values ± SEM; n = 3.
