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WNT5A-mediated miR-374a-5p affected VSMC phenotypic transformation (A) WB detection of intracellular WNT5A signal expression. (B) IF double-staining to detect the expression of smooth muscle cell contraction markers (SM22α and α-SMA). Scale bar = 25 μm. (C) WB detection of SM22α, α-SMA, Calponin, MMP1, MMP3, MMP9, TIMP3, and TIMP4 expression. (D) ELISA measurement of TNF-α, IL-1β, IL-6, and IL-10 levels. *P < 0.05 vs. inhibitor NC. &P < 0.05 vs. miR-374a-5p inhibitor + Vehicle. n = 3.
