FIG. 2.

(A) Five rpsL operon-containing plasmids used to analyze protein interactions in vivo. All the plasmids have the wild-type rpsL gene under the control of the ant promoter from phage P22. The common DNA sequence surrounding the ant promoter is given. RBS, ribosome binding site. The difference between the plasmids is the number or the position of operators. pSingle has a single hix operator, pDouble1, pDouble2, and pDouble3 have two operators, and pTriple has three operators. (B) DNA sequences of the two hix sites used as operators. These two hix sequences differ only in the central 2 bp (bold), preventing inversion. (C) Schematic representation of the Hin-producing plasmid pHinWT. This plasmid was derived from pBluescript II SK⁺.