Table 2.
Detection of cellular state in ferroptosis.
| Reagent | Reaction principle | Detection method | Variation | Ref. |
|---|---|---|---|---|
| CCK8/MMT | Reagent is reduced to blue purple crystal methyl black by succinate dehydrogenase in the mitochondria of living cells | Microplate plate analyzer, Microplate reader | Decline | [92] |
| Trypan blue dye | Trypan blue dye can enter inactivated cells or incompleted cell membranes | Microscope | Increase | [[93], [94], [95]] |
| Hoechst33342 and SYTOX Green | SYTOX Green marks the nucleic acid of dead cells, and Hoechst33342 marks the DNA of living cells | Fluorescence microscope, fluorescence microplate reader, flow cytometer | Green fluorescent increase, blue fluorescent decline | [54] |
| PI | PI is a small flurescent molecule, can enter the cell and bind to DNA | Fluorescence microscope, Flow cytometer | Increase | [96,97] |
| LDH | The plasma membrane ruptures and LDH is released | Microplate reader | Increase | [[98], [99], [100]] |
| CellTiter-Glo Luminescent Assay Kit | Content of ATP can reflect cell activity | Luminometer, Liquid scintillation meter | Decline | [101] |