. 2023 Dec 13;10(1):e23507. doi: 10.1016/j.heliyon.2023.e23507

Table 5.

Detection of various reaction products in ferroptosis.

	Reagent	Reaction principle	Detection method	Variation	Ref.
Fe²⁺	FerroOrange, PGSK, FeRhoNox-1	Increase of intracellular Fe²⁺ will trigger ferroptosis	Confocal microscope, Fluorescence microscope, Flow cytometer	Fluorescence enhancement	[[157], [158], [159]]
Fe²⁺	Iron Assay Kit	Increase of intracellular Fe²⁺ will trigger ferroptosis	Microplate reader	Increase	[145]
ROS	DCFH-DA, DHE	ROS leads to oxidative stress	Confocal microscope, Fluorescence microscope, Flow cytometer	Fluorescence enhancement	[109,160,161]
RNS	DAF-FM Diacetate	NO mediate lipid peroxidation	Confocal microscope	Fluorescence enhancement	[61]
RNS	HKYellow-1, BTMO-PN	ONOO- mediate lipid peroxidation and lipid peroxidation	Confocal microscope, Flow cytometry, Fluorescence microscope	Fluorescence enhancement	[61,162]
Lipid peroxidization products	C11-BODIPY	Lipid peroxidation is increased in ferroptosis	Fluorescence microscope, fluorescence microplate reader, flow cytometer	Increase	[163]
	MDA Assay Kit	MDA is main lipid peroxidization products	Microplate reader	Increase	[164]
	4-HNEAssay Kit	4-HNE is main lipid peroxidization products	Microplate reader	Increase	[164]
	TBARS	TBARS can detect MDA and some minor related compounds	Microplate reader	Increase	[165]