TABLE 1.
Disruption and complementation analysis of the yiaE gene
| Straina | 2KR activity (U/mg of protein) | Conversion of 2KDG to d-gluconateb | Growth on a 2KDG platec |
|---|---|---|---|
| JC7623 | 0.042 | + | − |
| JC7623 (yiaE::Km) | 0 | − | − |
| W3110 | 0.009 | + | + |
| W3110 (yiaE::Km) | 0 | − | − |
| W3110 (yiaE::Km)(pHD2) | 0.390 | + | + |
a
Strains were cultured for 18 h at 37°C in LB. Cells were then harvested, and 2KR activity was measured by using 2KDG as a substrate.
b
Conversion was done with crude cell extracts and the reaction products were determined by HPLC, as described in Materials and Methods.
c
M9 medium with 2KDG (5 g/liter) as the carbon source.