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Specific 2KR activity in E. coli W3110 cellsa
| Carbohydrate (1.0%) | Sp act of 2KR (U/mg of protein) |
|---|---|
| None | 0.012 |
| d-Glucose | 0.027 |
| d-Gluconate | 0.027 |
| 2KLG | 0.014 |
| 2KDG | 0.021 |
| 25DKG | 0.017 |
| 5KDG | 0.013 |
Strains were cultivated in LB containing 1.0% carbohydrate for 20 h at 37°C. Cells were then harvested, and 2KR activity was measured by using 2KDG as a substrate.
