TABLE 1.
E. coli strains and plasmids
| Strain or plasmid | Origin | Genotype | Source or reference |
|---|---|---|---|
| Strains | |||
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR | 30 | |
| DW35 | DW31 | zjd::Tn10 Δ(frdABCD)18 sdhC::kan araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR | 36 |
| HB101 | supE44 hsdS20(rB− mB−) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 | 26 | |
| Plasmids | |||
| pSDH15 | pBR322 | PSDHsdhC+D+A+B+ Ampr | 33 |
| pGC1002 | pBR322 | PFRDfrdA+B+C+D+ Ampr | 4 |
| pH3 | pBR322 | PFRDfrdA+B+C+D+ Ampr | 3 |
| pH3-177 | pACYC177 | PFRDfrdA+B+C+D+ Ampr | This study |
| pFGS | pBR322 | PFRDsdhC+(GTG)D+A+B+ Ampr | This study |
| pFGS-177 | pACYC177 | PFRDsdhC+(GTG)D+A+B+ Ampr Kanr | This study |
| pFAS | pBR322 | PFRDsdhC+(ATG)D+A+B+ Ampr | This study |