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. 2023 Dec 15;15(12):6701–6717.

Figure 2.

Figure 2

The effect of RPPH1 knockdown in MDA-MB-231 and HeLa cells. A. Detection of RPPH1 expression in untransfected mock cells, vector control, and RPPH1 knockdown clones HeLa-shRPPH1-1-39, HeLa-shRPPH1-1-52, MDA-MB-231-shRPPH1-1-72 and MDA-MB-231-shRPPH1-1-78. One-step RT-PCR was performed with 0.5 μg of total RNAs isolated from cells as indicated. B. Flowchart of study design. HeLa-shRPPH1-1-52, MDA-MB-231-shRPPH1-1-72 and mock cells were deep-sequenced for RNA expression and analyzed using bioinformatics databases. Significantly down-regulated genes with false discovery rate < 0.05 and fold change < 0.65 were further analyzed using bioinformatics tools and confirmed using experimental evidence.