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Several amino acid pathways and tRNA production are down-regulated in RPPH1 knockdown cells. A. The metabolic pathways of 40 down-regulated DEGs in RPPH1 knockdown cells were analyzed using MetaCore bioinformatics tools. Red arrow indicates the pathway associated with amino acid metabolism. B. RPPH1 knockdown cells of tRNA expression in HeLa and MDA-MB-231 cells. One-step RT-PCR was performed with 0.5 μg of total RNAs isolated from cells as indicated.
