表1.

肺癌内菌群相关研究及主要发现

Authors, Year	Sample size	Main findings
Yu G, et al. 2016[24]	31 (lung tumor)+ 31 (non-malignant lung tissue)	1. α diversity was significantly higher in non-malignant lung tissue than in tumor tissue. 2. Compared with squamous cell carcinoma, tissues of adenocarcinoma had increased relative abundance ofThermus and decreased relative abundance ofRalstonia.
Greathouse KL, et al. 2018[21]	1255 (lung tumor)+ 1256 (non-tumor adjacent tissue) (from databases)	1. There were distinct microbial populations within squamous cell carcinoma and adenocarcinoma. 2. Among squamous cell carcinoma, Acidovorax was more abundant in smokers and patients with TP53mutation.
Peters BA,et al. 2019[20]	19 (lung tumor)+ 19 (remote normal lung tissue)	1. Lung cancer tissue had lower richness and diversity than, but compositionally similar to paired normal lung tissue. 2. Families Koribacteraceae and Lachnospiraceae in lung cancer tissue were associated with reduced RFS and DFS.
Nejman D,et al. 2020[2]	245 (lung tumor)+ 231 (normal tissue)	1. The bacterial load of lung cancer was at an intermediate level among pan-cancer. 2. Compared with other tumor types, metabolic pathways that degrade chemicals in cigarettes were enriched in the bacterial community of lung cancer. 3. Several metabolic pathways related to the biosynthesis of metabolites that can be used by plants were enriched in bacteria of lung cancer of smokers.
Wong LM,et al. 2020[39]	930 (lung tumor)+ 930 (adjacent normal tissue) (from databases)	Age and gender were important factors affecting the bacterial composition in lung cancer.
Patnaik SK,et al. 2020[19]	48 (lung tumor)+ 48 (adjacent non-tumor lung tissue)	Due to the low abundance of bacteria in tumors and adjacent tissue, the results were susceptible to contamination and the authors did not compare the bacteria in these two types of samples.
Leblond N,et al. 2021[22]	29 (lung tumor)+ 29 (healthy adjacent tissue)	Enteric, potentially pathogenic and pro-inflammatory bacteria were more frequently found in cancer than healthy tissue.
Leng Q, et al. 2021[40]	31 (lung tumor)+ 31 (noncancerous lung tissue)	Compared with noncancerous lung tissues,Acidovorax was overrepresented in squamous cell carcinoma tissues and Capnocytophaga DNA was enriched in adenocarcinoma tissues.
Rolle A, et al. 2022[18]	12 (lung tumor)+ 12 (adjacent normal region)	1. In tumor microenvironment, bacterial burden was significantly higher in tumor cells compared with other types of cells. 2. Genes involved in the Wnt/β-catenin, hypoxia, and angiogenesis pathways showed a strong positive correlation with bacterial burden.

RFS: recurrence-free survival; DFS: disease-free survival.