TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype and other relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| MV10 | thr-1 leuB6 lacY1 thi-1 tonA21 supE44 rfbD1 ΔtrpE5 λ− | 37 |
| MV10Δlac | MV10 Δ(argF-lac) deoC1::Tn10 (Tetr) | 37 |
| MC1061K | F−araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac) X74 rpsL (Strsr) hsdR2 rK mK+mcrA mcrB1 Kmr | 32 |
| RR1 | F− Δ(gpt-proA)62 leuB6 supE44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 | 27 |
| DH5 α | F−endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZY -argF)U169 deoR [80dlacΔ(lacZ)M15] | 23 |
| DPB635 | Wild type | 6 |
| DBP636 | DPB635 topA66 | 6 |
| Pseudomonas aeruginosa | ||
| PAO1161Rif | Spontaneous Nalr Rifr derivative of PAO1161 | 5a |
| PAC452Rif | Spontaneous Rifr derivative of PAC452 | 31 |
| Plasmids | ||
| pCE61 | Derivative of RK21382 with parCBA and parDE deletions | 12a |
| pCE61-2.3 | pCE61 containing parCBA | 12a |
| pCE61-91 | pCE61 containing parA | This work |
| pCE61-92 | pCE61 containing parAB | This work |
| pCE61-94 | pCE61 containing parAC | This work |
| pCE61-95 | pCE61 containing parBC | This work |
| pVW8703 | pUC8Cm containing traFG | 42 |
| pAL4000 | RSF1010 derivative with promotorless luciferase gene | 16 |
| pTD10 | pAL4000 with parCBA promoter | 12 |
| pEKA28 | IncQ (RSF1010) pMHL3 derivative | 36b |
| pEKA30 | Derivative of pEKA28 containing Cre | 36b |
| pEKA28G | Gentamicin-resistant derivative of pEKA28 | This work |
| pEKA30G | Gentamicin-resistant derivative of pEKA30 | This work |