TABLE 3.
Use of a parCBA promoter and a luciferase fusion construct to measure ParA levels provided by various deletion constructs
| Plasmids | Stabilization region | Luciferase unitsa of E. coli
|
|
|---|---|---|---|
| RR1 | MV10 | ||
| pCE61 + pAL4000 | None | 251 | 131 |
| pCE61 + pTD10 | None | 2,024 | 1,443 |
| pCE61-2.3 + pTD10 | parCBA | 619 | 383 |
| pCE61-91 + pTD10 | parA | 652 | 422 |
| pCE61-92 + pTD10 | parBA | 491 | 473 |
| pCE61-94 + pTD10 | parCA | 164 | 950 |
| pCE61-95 + pTD10 | parCB | 2,092 | 2,693 |
a
Luciferase units were calculated as (reading 1 + reading 2)/200(optical density at 600 nm).