TABLE 4.
Effect of insertion of the multimer resolution systems of plasmids P1 and ColE1 on the stable maintenance of RK2 with deletions of the par region
| Plasmid(s) | Resolvase system | Rate of loss (% per generation)a with strain
|
||
|---|---|---|---|---|
| MC1061K | MV10Δlac | RR1b | ||
| pCE61 + pEKA28G | None | 1.50 | 0.45 | 2.71 |
| pCE61 + pEKA30G | loxP/Cre | 0.13 | 0.02 | 0.14 |
| pCE61 cer | cer | 1.24 | 0.83 | 0.05 |
a
The number of generations ranged from 75 to 90 in LB medium at 30°C. Similar results were obtained when the experiment was repeated.
b
Plasmids pEKA28 and pEKA30 were used in trans in this strain instead of plasmids pEKA28G and pEKA30G.