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Expression of T7-ExbB and T7-ExbD. T7 epitope-tagged proteins produced from ΔexbBD cells (KP1269) expressing T7-ExbB-ExbD (pKP339-pKP360), T7-ExbD-ExbB (pKP323-pKP361), or vector-only controls [pACYC184-pET-24(a)+] are shown. Equivalent numbers of cells were electrophoresed on SDS–11% polyacrylamide gels and detected by immunoblotting with a T7 epitope tag-specific monoclonal antibody.
