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. 2023 Dec 21;14:1295096. doi: 10.3389/fphar.2023.1295096

TABLE 1.

Sequences of primers used in real-time RT-PCR.

Gene name Polarity Primer sequence (sense and anti-sense) (5′ → 3′) Annealing temperature (°C)
Nuclear factor erythroid-2-related factor 2 (Nrf2) Sense CAG​CAT​AGA​GCA​GGA​CAT​GGA​G 60°C
Anti-sense GAA​CAG​CGG​TAG​TAT​CAG​CCA​G
Superoxide dismutase 2, mitochondrial (Sod2) Sense TAA​CGC​GCA​GAT​CAT​GCA​GCT​G 60°C
Anti-sense AGG​CTG​AAG​AGC​GAC​CTG​AGT​T
Heme oxygenase 1 (Hmox-1) Sense CAC​TCT​GGA​GAT​GAC​ACC​TGA​G 60°C
Anti-sense GTG​TTC​CTC​TGT​CAG​CAT​CAC​C
Transient receptor potential cation channel subfamily V member 1 (Trpv1) Sense ACC​ACG​GCT​GCT​TAC​TAT​CG 60°C
Anti-sense TCC​CCA​ACG​GTG​TTA​TTC​AG
Cyclophilin (Ppia) Sense GCTTTTCGCCGCTTGCT 60°C
Anti-sense CTCGTCATCGGCCGTGAT