Table 2.
CD4+ αβ T cell markers (human).
| Type of T cell | Annotation tool | Feature set |
|---|---|---|
| Th1 | CellTypist^ | CCL5, CXCR3, and TBX21 |
| Data2Talk& | ND | |
| Flow panel# | TNFα, IFNγ, IL-2, CXCR3, and TBX21 | |
| Flow panel€ | CCR1, CCR5, CD3, CD4, CD8-, CD14-, CD19-, CXCR3, IFNγR1, IFNγR2, IL-12Rβ2, IL-18Rα, IL27Rα, STAT1, STAT4, T-bet, IFNγ, IL-2, TNFα, and TNFβ | |
| Th1/GZMK | scGate | GZMK, EOMES, and CRTAM |
| Th2 | Flow panel# | IL-4, IL-5, CCR4, and GATA3 |
| Data2Talk& | ND | |
| Flow panel€ | CCR3, CCR4, CCR8, CD3, CD4, CD8-, CD14-, CD19-, CXCR4, IL-4Rα, IL17, RB, ST2/IL-33R, TSLPR, GATA-3, IRF4, STAT5, STAT6, IL-4, IL-5, IL-9, IL-10, IL-13, and IL-21 | |
| Th9 | Flow panel# | IL-9, IL-10, and IRF4 |
| Flow panel€ | CD3, CD4, CD8-, CD14-, CD19-, IL-4Rα, IL-17RB, TGF-β RII, IRF4, PU.1, CCL17, CCL22, and IL-9 | |
| Th17 | scGate | IL17A, IL17F, RORC, CTSH, KLRB1, CCL20, and IL26 |
| CellTypist | IL7R, CCR6, and ZBTB16 | |
| Data2Talk& | ND | |
| Flow panel# | CCR6, CD161 (KLRB1), IL-17, IRF4, and RORγt (RORC) | |
| Flow panel€ | CCR4, CCR6, CD3, CD4, CD8-, CD14-, CD19-, IL-1RI, IL-6Rα, IL-21, IL-23, TGF-β RII, Batf, IRF4, RORα, RORγt/RORC2, STAT3, CCL20, IL-17A, IL-17F, IL-21, IL-22, and IL-26 | |
| Tfh or Th21 | scGate | IL21, CD200, CXCL13, TOX, and TOX2 |
| CellTypist^ | PDCD1, ICOS, and CXCR5 | |
| Data2Talk& | ND | |
| Flow panel# | IL-21 | |
| Flow panel€ | BTLA, CD3, CD4, CD8-, CD14-, CD19-, CD40 Ligand, CD57, CD84, CXCR4, CXCR5, ICOS, IL-6 R α, IL-21 R, CD10, OX40, PD-1 (PDCD1), SLAM, CD150, Bcl-6, c-Maf, STAT3, CXCL13, IFNγ IL-4, IL-10, IL-17A, IL-17F, and IL-21 | |
| Th22 | Flow panel# | IL-22, CCR10 |
| Flow panel€ | CCR4, CCR6, CCR10, CD3, CD4, CD8-, CD14-, CD19-, CD161-, IL-6Rα, TGF-β RII, TNFRI, AHR, Batf, STAT3, CCL7/MCP-3, CCL15/MIP-1δ, FGFs, IL-10, IL-13, IL-21, IL-22, and TNF-α | |
| Regulatory T cells | scGate | FOXP3 |
| CellTypist^ | CTLA4, IL2RA, and FOXP3 | |
| Flow panel€ | CD73, CD3, CD4, CD5, CD14-, CD19-, IL-2Rα, ENTPD1, CD103, IL-7Rα low, CCLA-4, Folate Receptor 4, GITR, CD223, LAP, GARP, BDCA-4, CD134, CD62L, FOXP3, Helios(+/−), STAT5, Galectin-1, IL-10, IL-35, and TFG-β | |
| PD-1+ Tem/Effector Th | CellTypist^ | PDCD1, CD4, and CTLA4 |
| Tcm/Effector Th | CellTypist^ | CD4, CCR7, and SELL |
| Memory CTL | CellTypist^ | GZMK, CD4, and IL10 |
| Tem/Effector Th | CellTypist^ | KLRB1, AQP3, and ITGB1 |
| Naive | Literature (27) | CD25− (IL2RA), CD45RA, CD45RO−, and CD127 |
| Teff | Literature (27) | CD25, CD45RA(+/−), CD45RO(+/−), and CD127- |
| Tem | Literature (27) | CD25−, CD45RA−, CD45RO, and CD127 |
| Tcm | Literature (27) | CD25, CD45RA−, CD45RO, and CD127 |
| MAIT | Literature (28) | TRAV1-2 and CD161 (KLRB1), and IL-18Ra |
Th, T helper; NK, Natural Killer; CTL, Cytotoxic T cells; Tfh, Follicular helper T cells; Tcm, Central memory; Tem, Effector memory.
#Flow cytometry protein expression panel markers from: https://www.biocompare.com/Editorial-Articles/569888-A-Guide-to-T-Cell-Markers/
€Marker summary from https://www.rndsystems.com/resources/cell-markers/immune-cells
&Bioturing can predict cell types based on 80,574,317 cells https://talk2data.bioturing.com/predict
^Curated markers from CellTypist (V2 list of markers).
ND, Not disclosed.