Table 5.
Miscellaneous T cell markers (human).
| Type of T cell | Annotation tool | Feature set |
|---|---|---|
| ETP | CellTypist^ | ACY3, CD34, and SPINK2 |
| DN thymocytes | CellTypist^ | FXYD2, HES1, and CD99 |
| Treg(diff) | CellTypist^ | CD27, CCR7, and IKZF2 |
| T(agonist) | CellTypist^ | MIR155HG, BIRC3, and SMS |
| Early activation | Flow panel% | CD69 |
| Later activation | Flow panel% | CD25 (IL2RA) |
| Very late activation | Literature (33) and flow Panel% | HLA-DR% (HLA-DRA, HLA-DRB5, and HLA-DRB1), CD38 |
| Senescence | Literature (33) | CD57 (B3GAT1) |
| Literature (34) | CD57 and KLRG1 | |
| Exhaustion | Literature (33) | PD1 (PDCD1) |
| Literature (35) | TIGIT, CD279, LAG3, and PDCD1 | |
| Literature (36) | Transcription factors panel of markers: TOX, NR4A, T-bet, EOMES, NFAT, IRF4, and BATF Inhibitory receptors: PD-1 (PDCD1), LAG-3 and HAVCR2 (TIM-3) |
|
| Literature (29)* | HAVCR2, PDCD1, LAYN, TOX, ITGAE, CTLA4, LAG3, ENTPD1, TIGIT, and CXCL13 | |
| Cycling T cells | CellTypist^ | MKI67, TOP2A, and CD3D |
| Proliferation | Literature (35) | MKI67 and TYMS |
| Literature (29)* | ASPM, TOP2A, UBE2C, MKI67, CDKN2A, CD70, CDK4, and CDK6 |
DN, Double negative; ETP, Early thymic progenitors; Treg, regulatory T cell.
^Curated markers from CellTypist (V2 list of markers).
%Flow cytometry protein expression panel markers from: https://www.sartorius.com/en/applications/life-science-research/cell-analysis/flow-cytometry/immune-cell-function/t-cell-activation
&Bioturing can predict cell types based on 80,574,317 cells https://talk2data.bioturing.com/predict
^Curated markers from CellTypist (V2 list of markers).
*Based on Figure 1 top associated markers from a single-cell study.