Fig. 7.

The neuroprotective effect of ChemR23 against OGD-induced neuronal pyroptosis was NLRP3 dependent. A Representative immunofluorescent images of NLRP3 in cultured neurons. Scale bar = 20 μm. B, C Immunoblots and quantitative analysis of NLRP3, ASC, caspase-1 p20, GSDMD-N in treated neurons after OGD. D Representative scanning electron microscopy pictures of neurons. Scale bar = 10 μm. E LDH release was assessed and quantified in neurons subjected to OGD for 4 h. F, G ELISA analysis for IL-1β and IL-18 levels in supernatants of neuron subjected to OGD for 4 h. At least three independent experiments were repeated. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001