Fig. 2.

Agarose gel visualization of amplified 18S rRNA gene region of Plasmodium spp. a PCR products of amplified with primers rPLU1/rPLU5 for the 18S rRNA gene region of Plasmodium spp. M, 100 bp ladder; N, negative control (distilled water); P, standard positive controls (P. falciparum); lane 1, positive field sample; lanes 2–10, negative field samples. b Nested PCR products of amplified with primers rPLU3/rPLU4 for the 18S rRNA gene region of Plasmodium spp. M, 100 bp ladder; N, negative control (distilled water); P, standard positive controls (P. falciparum); lanes 1–6, positive field samples; lanes 7–10, negative field samples. c Nested PCR products of amplified with primers rFAL1/rFAL2 for the 18S rRNA gene region of P. falciparum. M, 100 bp ladder; N, negative control (distilled water); P, standard positive controls (P. falciparum); lanes 1–6, positive field samples; lanes 7–10, negative field samples