Fig. 9.

TREM1 modulates the activation of the NLRP3 inflammasome via the NF-κB pathway in BV2 cells. After transfection with either overexpression negative control vector or overexpression TREM1 (oeTREM1) for 24 hours, the cell culture medium was replaced and subsequently treated with PDTC (100 μM) for a period of 24 hours prior to harvesting. Immunoblots were conducted on BV2 cells, including the vector, vector-PDTC, oeTREM1, and oeTREM1-PDTC groups, to assess TREM1, p-p65 to p65 ratio and p-IκBα to IκBα ratio (a-e). Immunoblots were also performed to assess the expression levels of NLRP3, cleaved-caspase-1, ASC, mature IL-1β, and mature IL-18 (f-k). Mean ± SEM values are presented. One-way ANOVA and Tukey's post hoc tests; ***p < 0.001 vs. the vector group and ^###p < 0.001 vs. the oeTREM1 group