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. Author manuscript; available in PMC: 2024 Jan 5.
Published in final edited form as: J Med Chem. 2023 Aug 21;66(17):11831–11842. doi: 10.1021/acs.jmedchem.3c00406

Figure 8.

Figure 8.

A) In the pRXG(UGA) reporter plasmid, the fused in-frame rfp-gfp genes are separated by a stop codon UGA. Stop codon readthrough is required for expressing the GFP activity. B) Drop diffusion test on agar plate inoculated with the E. coli cells carrying the pRXG(UGA) reporter plasmid. Two μL droplets of 0.2 mM solutions of compounds 3, 27 and 29 or of the positive control Api-137, and 1 μL drop of a 1 mg/mL solution of a negative control antibiotic chloramphenicol that does not induce stop codon readthrough, were applied to the plates. Shown is the false-colored image of the plate imaged in the Cy2 channel after overnight incubation at 37 °C.