TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Genotype or phenotype | Origin or constructiona |
|---|---|---|
| Strains | ||
| C600 | supE44 thi-1 thr-1 leuB6 lacY1 tonA21 hsdR | Laboratory collection |
| UM21 | HfrC metB1 relA1 spoT1 tolZ21 sfhC | 24 |
| PhB782 | As UM21 hflB+zha-b::Tn10/pSC101lacIq | Laboratory collection |
| YYC188 | secA214 Δ(aroP-aceF)73 fhuA54? lacZ608(Am) λcI857 zbj-927::Tn10 poxB11 pflB1 pps-4 rpsL104 thi-1 | Mary Berlyn, CGSC6776 (7) |
| RE103 | proA23 lac-28 trp-30 his-51 rpsL101 cmlA1 | Mary Berlyn, CGSC4698 (25, 26) |
| RC712 | proA23 lac-28 trp-30 his-51 rpsL101 | Mary Berlyn, CGSC4413 |
| PhB1001 | As RE103 (cmlA1) zbj-927::Tn10 | RE103 + P1/YYC118 |
| PhB631 | From FB8 (prototroph) | Laboratory collection |
| PhB1010 | As PhB631 zbj-927::Tn10 | PhB631 + P1/PhB1001 |
| PhB1011 | As PhB631 zbj-927::Tn10 cmlA1 | PhB631 + P1/PhB1001 |
| Plasmids | ||
| pGB2 | From pSC101; aadA | 8 |
| pSC101lacIq | From pSC101; aadA lacIq | 28 |
| pCTCIII | From pACYC184; Ptac-λcIII | 23 |
| pIL1991 | From pUHE21; lactococcal phage bIL66 M operon | 3 |
| pKSCMLA | From pKS; cmlA | This work |
| pBRCMLA | From pBR322; cmlA | This work |
a
Isogenic pairs of strains were constructed by P1 vir-mediated transductions (20).