Figure 4.

Sirt2i modulates super-enhancer landscapes in Atrx− mNPCs: (A-B) Single cell nuclear chromatin condensation (CCP) analysis demonstrating reduced chromatin condensation in Atrx-deficient mNPCs with more modest effects in Atrx + counterparts. (C) Venn diagrams showing shared and unique ChIP-seq peaks for enhancer-associated H3K4me1 and H3K27ac and repressive H3K27me3 marks in Atrx + and Atrx− mNPCs deficient NPCs. Hypergeometric P values are shown. (D) Rank Ordering of Super-Enhancers (ROSE) analysis for H3K27ac showing larger increases in super-enhancer number following 1 µM AGK2 treatment in Atrx− mNPCs than in Atrx + mNPCs. (E-F) GSEA correlations for all gene sets significantly associated with Sirt2i-mobilized super-enhancers in Atrx + and Atrx− mNPCs. Where applicable, error bars reflect SEM; P values determined by unpaired, two-tailed t-test (*P < 0.05)