Figure 10.

Kv2 channel expression and localization in human DRG neurons is similar to mice. A, Immunofluorescence from human DRG neurons labeled with anti-Kv2.1 and anti-Kv2.2 antibodies. Autofluorescence attributed to lipofuscin is labeled in right panel while apparent Kv2.1 and Kv2.2 protein are labeled in left and middle panel respectively. Scale bar is 50 μm. B, Z-projection of anti-Kv2.2 (left) and anti-NF200 immunofluorescence (middle) of human DRG neuron somata. Green arrow head indicates asymmetric distribution of Kv2.2 clusters on neuron soma, green arrow indicates the apparent stem axon. Scale bar is 20 μm. C, Z-projection of anti-Kv2.1 (upper left), anti-Kv2.2 (upper right) and anti-NF200 (lower left) immunofluorescence of a human DRG neuron. Magenta and green arrows indicate Kv2.1 and Kv2.2 respectively on the apparent stem axon. Inset shows expansion of dotted line boxes which highlights Kv2.1 and Kv2.2 clusters on the apparent stem axon. Autofluorescence attributed to lipofuscin is labeled in lower right panel. Scale bar is 50 μm. All images are from donor #1. Detailed information on each donor can be found in the Human Tissue Collection section of the methods.