Table 3.
Antibodies used for western blotting
| Primary Antibody | Antibody species | Blocking buffer | Dilution | Catalog number |
|---|---|---|---|---|
| p-STAT-1 | Rabbit | 5% BSA in TBST | 1:1000 | Cell Signaling 7649 |
| STAT1 | Rabbit | 5% BSA in TBST | 1:1000 | Cell Signaling 9172 |
| IRF3 | Rabbit | 5% milk in TBST | 1:1000 | Cell Signaling 4302 |
| IFIT1 | Rabbit | 5% milk in TBST | 1:1000 | Cell Signaling 14769 |
| Viperin | Rabbit | 5% milk TBST | 1:1000 | Cell Signaling 13996 |
| MDA5 | Rabbit | 5% milk TBST | 1:1000 | Cell Signaling 5321 |
| p-PKR | Rabbit | 5% BSA in TBST | 1:1000 | Abcam 32036 |
| PKR | Rabbit | 5% milk TBST | 1:1000 | Cell Signaling 12297 |
| SARS-CoV-2 Nucleocapsid | Rabbit | 5% milk TBST | 1:2000 | Genetex GTX135357 |
| MERS-CoV Nucleocapsid | Mouse | 5% milk TBST | 1:2000 | Sino Biological 40068-MM10 |
| HCoV-229E Nucleocapsid | Rabbit | 5% milk TBST | 1:2000 | Sino Biological 40640-V07E |
| HCoV-NL63 Nucleocapsid | Rabbit | 5% milk TBST | 1:2000 | Sino Biological 40641-T62 |
| HRV-16 VP0/2 | Mouse | 5% milk TBST | 1:1000 | QED Biosciences 18758 |
| IAV HA | Mouse | 5% milk TBST | 1:2000 | Sigma H9658 |
| GAPDH | Rabbit | 5% milk or 5% BSA in TBST | 1:2000 | Cell Signaling 2118 |
| Secondary Antibody | Antibody species | Blocking buffer | Dilution | Catalog number |
| Anti-rabbit IgG HRP-linked | Goat | Same as primary | 1:3000 | Cell Signaling 7074 |
| Anti-mouse IgG HRP-linked | Horse | Same as primary | 1:3000 | Cell Signaling 7076 |