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[Preprint]. 2023 Dec 19:2023.12.19.572350. [Version 1] doi: 10.1101/2023.12.19.572350

Fig. 1. CDCA7 and HELLS accumulate on chromatin upon inhibition of maintenance DNA methylation.

Fig. 1

(A) X. laevis sperm nuclei were incubated with interphase egg extracts depleted with mock IgG (ΔMOCK), anti-DNMT1 (ΔDNMT1), anti-HELLS (ΔHELLS), or anti-CDCA7e (ΔCDCA7e) antibodies for 3 h in the presence of cycloheximide. Chromatin was isolated and analyzed by western blotting. (B) X. laevis sperm nuclei were isolated at indicated time points after incubation with interphase Xenopus egg extracts in the presence or absence of 0.5 µM mouse DPPA3 (mDPPA3), a protein that inhibits binding of UHRF1 and DNMT1 to chromatin. Chromatin-associated proteins were analyzed by western blotting.