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[Preprint]. 2023 Dec 19:2023.12.19.572350. [Version 1] doi: 10.1101/2023.12.19.572350

Fig. 5. CDCA7 recruits HELLS to hemimethylated DNA.

Fig. 5

(A) Beads coated with unmethylated or hemimethylated DNA (pBluescript) were incubated with interphase Xenopus egg control mock IgG-depleted extracts (ΔMOCK) or CDCA7e-depleted extracts (ΔCDCA7e) for 30 min. Beads were isolated and analyzed by western blotting. (B) 35S-labeled HELLS or HELLS Δ63–96 was incubated with beads coated with 200 bp unmethylated or hemimethylated DNA. Beads were isolated and associated 35S-labeled proteins were visualized by autoradiography. Nonspecific DNA-binding protein Xkid DNA-binding domain (Xkid-DBD) was used as a loading control.