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[Preprint]. 2023 Dec 19:2023.12.19.572350. [Version 1] doi: 10.1101/2023.12.19.572350

Fig. 6. CDCA7e and HELLS regulate replication-uncoupled maintenance DNA methylation.

Fig. 6

(A) Xenopus sperm nuclei were incubated for 120 min in interphase Xenopus egg extract in the presence of 0.5 µM recombinant mDPPA3. Chromatin was isolated and reincubated in interphase egg extract in the presence or absence of 150 μM aphidicolin (APH). (B, C). Sperm nuclei were incubated for 120 min in Mock-depleted extracts or either CDCA7e-depleted (B) or HELLS-depleted (C) extracts supplemented with mDPPA3. Chromatin was isolated and reincubated in Mock-depleted and either CDCA7e-depleted (B) or HELLS-depleted (C) extracts in the presence of aphidicolin. Chromatin was then isolated at indicated time points and chromatin-bound proteins were analyzed by western blotting using indicated antibodies.