Extended Data Fig. 6 |. Comparison of 5-HT2A lisuride cryoEM structure and crystal structure.

Overlay of the lisuride cryoEM structure solved in this work and the previously published 5-HT2A-lisuride crystal structure (PDB ID: 7WC7). There are some differences within the orthosteric pocket, but small shifts are noticed between the two structures. W151^3.28 (orange circle) shows a small shift inward, but also follows the slight shift of lisuride within the binding pocket. Additionally due to the penetration of a lipid moiety (blue circle) into the orthosteric pocket, F234^5.38 also was changed significantly (black circle). The pose found in the crystal structure more closely resembles the predicted structure from AF, indicating it may be sampling this space.