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[Preprint]. 2024 Mar 13:2023.12.20.572662. Originally published 2023 Dec 21. [Version 2] doi: 10.1101/2023.12.20.572662

PMC10769324.1; 2023 Dec 21
PMC10769324.2; 2024 Mar 13

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Extended Data Figure 13 — Comprehensive processing flow for the Z7757 structure. After 2D-classification and several rounds of 3D classification in cryoSPARC, the particles were transferred to for no-alignment 3D-classification focused on the receptor. Once a good consensus set of particles was identified and a further NU-refinemnet carried out, Bayesian polishing was performed on the particle set. A focused refinement was done on the receptor and a consensus map generated using Chimera. FSC plots for the receptor and heterotrimer as well as an angular distribution plot are also shown.