Skip to main content
[Preprint]. 2024 Feb 5:2023.12.22.573054. Originally published 2023 Dec 22. [Version 2] doi: 10.1101/2023.12.22.573054

Figure 2. Cyfip2 acutely regulates branched actin polymerization and NMDARs to establish the acoustic startle threshold.

Figure 2.

(A) Sensitivity indices for 5 dpf cyfip2 sibling (+/) and mutant (−/−) larvae, following a 40-minute heatshock at 120 hpf (5 dpf) to express either wildtype (Tg+; green), Rac1− (ΔRac1+; blue) or FMRP/eIF4E- (ΔFMRP+; pink) binding deficient versions of Cyfip2-EGFP. Comparisons were made to non-transgenic (Tg−), heatshocked sibling (+/) and mutant (−/−) controls. All indices (mean ± SD) compared using a Kruskal-Wallis test with Dunn’s multiple comparisons correction; p-values listed; p** < 0.01, p**** < 0.0001. (B) Sensitivity indices for 5 dpf cyfip2 wildtype (+/+; white bar) and heterozygous (+/−; gray bar) larvae, treated for 30 minutes on d5 with 5, 20 or 50 μM CK-869. Comparisons were made both within genotype and within condition. All indices (mean ± SD) compared using a Kruskal-Wallis test with Dunn’s multiple comparisons correction; p* < 0.05; p**** < 0.0001. (C) Sensitivity indices for 5 dpf Tüpfel longfin (TLF) larvae treated for 30 minutes on d5 with the highest, non-lethal doses the formin antagonist (SMIFH2; 5 μM), PAK3 antagonist (IPA-3; 50 μM) and ROCK antagonist (GSK429286; 100 μM). Comparisons were made between respective treatments and the DMSO controls. All indices (mean ± SD) were compared using a Kruskal-Wallis test with Dunn’s multiple comparisons correction; All comparisons made were non-significant (n.s.). (D) Sensitivity indices for 5 dpf cyfip2 sibling (+/) and mutant (−/−) larvae, treated for 30 minutes on d5 with 100 or 500 μM MK-801. Comparisons were made both between genotypes within condition and between conditions by genotype. All indices (mean ± SD) were compared using an Ordinary one-way ANOVA with Tukey’s multiple comparisons correction. p** < 0.01; p*** < 0.001; p**** < 0.0001.