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[Preprint]. 2023 Dec 23:2023.12.21.572835. [Version 1] doi: 10.1101/2023.12.21.572835

Figure 2. PDE breaks the Parascaris germline chromosome and silences germline-expressed genes.

Figure 2.

A. Features of the Parascaris X chromosome illustrated in a circos plot. Descriptions of the features from outer to inner circles: 1). Retained (dark blue) and eliminated DNA (red); the eliminated regions are highlighted in red across all circles; 2). The sex chromosome region (yellow bar); 3–4). Genomic (Illumina) read coverage from testis (red) and male intestine (light blue); 5). GC content (purple) and the average GC line; 6). Repeat density (light green) and the average repeat line; and 7–12). Expression (normalized RNA-seq) data for male mitotic region (light blue), male meiotic region (blue), ovary (pink), mixed embryos (orange), intestine (green), and carcass (dark green). B. Eliminated regions have higher GC content. C. Eliminated DNA contains more repeats. D. Eliminated genes are only expressed in the germline - mainly in the testis. The heatmap shows 743 expressed eliminated genes (rpkm >= 5 in at least one of the tissues; see Table S1). P-values for B and C were determined using Welch’s t-tests (*** P < 0.01 and **** P < 0.001).