TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| BSN1 | M182 hns::Cmr | 16 |
| DK2179 | MC4100 φ42-1 lamB-lacZ | 31 |
| BDG100 | MC4100 φ42-1 lamB-lacZ hns::Cmr | This study |
| JGJ100 | MC4100 φ42-1 lamB-lacZ stpA60::Kmr | This study |
| JGJ101 | MC4100 φ42-1 lamB-lacZ hns::CmrstpA60::Kmr | This study |
| BSN5 | MC1061 stpA60::Kmr | 32 |
| pop2481 | MC4100 malT′-lac′ZYA | 24 |
| BEU603 | Δhns trp::Tn10 | 32 |
| JGJ102 | pop2481 trp::Tn10 | This study |
| JGJ103 | pop2481 trp::Tn10 Δhns | This study |
| JGJ104 | pop2481 stpA60::Kmr | This study |
| JGJ105 | pop2481 trp::Tn10 stpA60::Kmr | This study |
| JGJ106 | pop2481 trp::Tn10 Δhns stpA60::Kmr | This study |
| JGJ107 | MC4100 mlc | This study |
| JGJ108 | JGJ107 trp::Tn10 | This study |
| JGJ109 | JGJ107 trp::Tn10 Δhns | This study |
| JGJ110 | JGJ107 trp::Tn10 stpA60::Kmr | This study |
| JGJ111 | JGJ107 trp::Tn10 Δhns stpA60::Kmr | This study |
| MC4100 | rpoS+ Δ(argF-lac) | 8 |
| BSN26 | MC4100 trp::Tn10 | This study |
| BSN27 | MC4100 trp::Tn10 Δhns | This study |
| BSN28 | MC4100 trp::Tn10 stpA60::Kmr | This study |
| BSN29 | MC4100 trp::Tn10 Δhns stpA60::Kmr | This study |
| BSN19 | MC4100 stpA60::Kmr | This study |
| Plasmids | ||
| pHA7* | crp* gene on pBR332 | 36 |
| pBR322 | Cloning vector | 6 |
| pJGJ101 | ′mlc′ fragment cloned into pCH257 | This study |
| pCH257 | Cloning vector | 15 |