FIG. 5.

Low-resolution mapping of the ctaB transcript 3′ terminus by RNase protection assays. In lane M, the size marker SequaMark (Research Genetics) was used by following the supplier’s protocol. Sizes in nucleotides (nt) are indicated on the left. The other lanes contained 1, 2, and 5 μg of total cellular RNA isolated from cells at T₀, hybridized to the long probe, and treated with 0.025 U of RNase A and 1 U of RNase T₁. Protected hybrids were separated by electrophoresis on a 5% polyacrylamide gel containing 8 M urea. An autoradiograph of the dried gel is shown. The structures of the long probe and the short probe are shown.