TABLE 2.
Strain and relevant genotype |
caa3 contenta in the following medium:
|
|
---|---|---|
Schaeffer’s | Schaeffer’s + glucose | |
RB1 (wild type) | Blue | White |
RB996 ΔctaA | White | White |
RB1298 ctaBCD′-lacZ CmrbctaBCDEFc | Blue | White |
RB1319A ctaBC′-lacZ CmrdctaCDEFc | White | White |
RB1320 ctaBC′-lacZ CmrdctaCDEFc | White | White |
RB1321 ctaBC′-lacZ CmrdctaCDEFc | White | White |
caa3 activity measured with TMPD as a substrate. Blue, caa3 positive; white, caa3 not detectable.
The chloramphenicol resistance cassette is transcribed in the same direction as ctaBCDEF.
Genotypes of integrants show the positions of chloramphenicol resistance cassette and lacZ fusion plasmid integration.
The chloramphenicol resistance cassette is transcribed in the direction opposite that of ctaBCDEF.