Fig. 3. Mitochondrial respiration is critical for nuclear accumulation of Hsp104.

a Schematic visualization of generation and analysis of the genome-wide deletion library endogenously equipped with Hsp104^GFP. Hits scored as “weaker nuclear accumulation” compared to control cells were subjected to further analyses. The number of deletion mutants in respective classes is given in brackets. b Visualization of the three most prominent enrichment clusters of hits classified as “weaker nuclear accumulation” compared to control cells after STRING analysis and Markov clustering. See Supplementary Fig. 3a for the complete STRING network. c Micrographs of wild type, cox12∆ and mip1∆ cells endogenously expressing Hsp104^GFP at 24 h. Scale bar: 2 µm. d Quantification of nuclear accumulation of Hsp104^GFP in cells as described in (c). Schematic drawings illustrate major phenotypes. N = Nucleus. Dot plots with mean (square) and median (line). Each dot represents one biological replicate (Ctrl.: 249 cells; cox12∆: 202 cells; mip1∆: 193 cells). e Micrographs of cells endogenously expressing Hsp104^GFP stained with Mitotracker CMXRos to visualize the mitochondrial transmembrane potential (mitoTMP) at 24 h (maximum intensity projections from Z-stacks). Scale bar: 2 µm. f Micrographs of cells endogenously expressing Hsp104^GFP cultivated in glucose or glycerol-containing media at 8 h. Schematic drawings illustrate major phenotypes. N = Nucleus. Scale bar: 2 µm. g Quantification of nuclear accumulation of Hsp104^GFP in cells as described in (f). Dot plots with mean (square) and median (line). Each dot represents one biological replicate (Glucose: 110 cells; Glycerol: 67 cells). h Micrographs of cells endogenously expressing Hsp104^GFP treated with antimycin A to inhibit mitochondrial respiration after 24 h. Scale bar: 2 µm. *p < 0.05; ***p < 0.001. Source data are provided as a Source Data file. See Supplementary Table 3 for details on statistical analyses.