Fig. 4. Nuclear Hsp104 interacts with translation-associated factors in aged cells.

a Schematic visualization of the workflow for proximity labeling (BioID) experiments to assess proximity to the bait protein (Hsp104 variants endogenously equipped with the biotin ligase BirA*). b Schematic visualization of the workflow for co-immunoprecipitation (CoIP) experiments to assess interaction with the bait protein (Hsp104 variants endogenously equipped with GFP). c VENN diagram to visualize hits shared between BioID and CoIP after performing STRING analysis, classified as “Translation-associated” and “Others”. d Listing of hits from (c) present in BioID and CoIP analyses.