PLC tissues were collected from patients to generate LICOB. In total, 65 organoids were established from 57 patients, including HCC (n = 44), ICC (n = 12), CHC (n = 4) and HB organoids (n = 5). Multi-omics analyses of these organoids were performed by using reduced representation bisulfite sequencing (RRBS), whole-exome sequencing (WES), RNA-seq, and label-free proteomics. The sensitivity of the organoids to 76 approved or investigational drugs was tested.