TABLE 3.
Mutational analysis of transcriptional activation at the tpl promoter
| Strain | Relevant genotype | β-Galactosidase activitya
|
|
|---|---|---|---|
| Glucose | Glycerol | ||
| SP1312 (Ptpl-lacZ+)mut1 | tyrR+, G→A in box A | 57.9 | 555 |
| SP1312 (Ptpl-lacZ+)mut2 | tyrR+, G→A in box B | 111.0 | 549 |
| SP1312 (Ptpl-lacZ+)mut3 | tyrR+, G→A and C→T in box C | 47.8 | 85.3 |
| SP1313 (Ptpl-lacZ+)mut3 | Δ(tyrR), G→A and C→T in box C | 22.0 | 22.6 |
| SP1312 (Ptpl-lacZ+)Δ64 | tyrR+, 64-nucleotide deletion | 41.2 | 278 |
| SP1313 (Ptpl-lacZ+)Δ64 | Δ(tyrR), 64-nucleotide deletion | 13.1 | 30.0 |
| SP1312 (Ptpl-lacZ+)Δ130 | tyrR+, 130-nucleotide deletion | 34.1 | 88.9 |
| SP1313 (Ptpl-lacZ+)Δ130 | Δ(tyrR), 130-nucleotide deletion | 17.1 | 55.2 |
| SP1312 (Ptpl-lacZ+)mut2,3 | tyrR+, G→A and C→T in box C, G→A in box B | 42.0 | 72.7 |
| SP1313 (Ptpl-lacZ+)mut2,3 | Δ(tyrR), G→A and C→T in box C, G→A in box B | 28.3 | 38.2 |
| SP1312 (Ptpl-lacZ+)Δ64C | tyrR+, 64-nucleotide deletion, box C is replaced by box A | 93.6 | 170 |
| SP1313 (Ptpl-lacZ+)Δ64C | Δ(tyrR), 64-nucleotide deletion, box C is replaced by box A | 12.1 | 28.7 |
| SP1312 (Ptpl-lacZ+)Δ179C | tyrR+, 179-nucleotide deletion, box C is replaced by box A | 65.9 | 72.9 |
| SP1313 (Ptpl-lacZ+)Δ179C | Δ(tyrR), 179-nucleotide deletion, box C is replaced by box A | 14.3 | 22.0 |
| SP1313 (Ptpl-lacZ+)Δ64C/pJC100 | Δ(tyrR) ptyrR+ Δ64C | 316 | 527 |
| SP1313 (Ptpl-lacZ+)Δ179C/pJC100 | Δ(tyrR) ptyrR+ Δ179C | 410 | ND |
| SP1313 (Ptpl-lacZ+)Δ179C/pJC100/pJC136 | Δ(tyrR) (ptyrR+) Δ179C | 614 | ND |
a
β-Galactosidase activity is expressed in Miller units. Cultures were grown in glucose or glycerol as indicated. ND, not determined.