Table 2.

qPCR amplification protocol.

Step	Temperature	Time
Holding Stage	95 °C	10 min
Cycling Stage (40 cycles)	95 °C	15 s
	60 °C	1 min
	72 °C	20 s
Melt Curve Stage	95 °C	15 s
	60 °C	1 min
	95 °C	30 s
	60 °C	15 s