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. 2023 Nov 23;300(1):105492. doi: 10.1016/j.jbc.2023.105492

Figure 5.

Figure 5

Enzyme expression and protein methylation in CRC cell lines treated with MTDIA and AG-270.A, comparison of seven CRC cell lines after 5 days treatment in culture. Western blotting was performed on two biological replicates. B, quantification and comparison of symmetric dimethyl-arginine (SDMA, p = 0.0021), asymmetric dimethyl-arginine (ADMA, p = 0.9487), and trimethyl-lysine (p = 0.8954) content normalized to GAPDH. Quantification performed in ImageJ. Data shown here are the quantification of representative blots shown in panel A. Significance determined with Student’s t test. C, correlation analysis of untreated bands in panel A. Pearson’s coefficient and coefficient of determination calculated in Graphpad Prism. D, comparison of protein changes in panel A after 5 days treatment in culture. CRC, colorectal carcinoma.