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. 2023 Nov 23;300(1):105492. doi: 10.1016/j.jbc.2023.105492

Figure 6.

Figure 6

Mechanism of cell growth inhibition induced by MTDIA and AG-270.A and B, representative cytometric traces analyzing cell cycle. Cells stained with propidium iodide (PI) and analyzed by Cellometer Spectrum fluorescent imager. Analysis performed in FlowJo (n = 4) and plotted in GraphPad prism. C and D, Representative flow cytometric gating analysis of WT HT-29 cells after 5 days treatment. Quantification of early (Q3) and late (Q4) apoptosis performed in FlowJo (n = 3) and plotted in GraphPad Prism. E, Western blotting of molecular markers of apoptosis in WT HT-29 cells after 5 days treatment. F, RT-PCR amplification of MDM4 full length (fl) and spliced (s) variants flanking exon 7 (top) and Western blotting of full-length MDM4, p53, and MDM2 (bottom) proteins in WT HT-29 cells.